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cell nuclei  (MedChemExpress)


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    Structured Review

    MedChemExpress cell nuclei
    Cell Nuclei, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hoechst+33258/Hoechst+33258/pm42387506-120-1-8
    Average 95 stars, based on 36 article reviews
    cell nuclei - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: Identification of a novel minor-groove DNA binder that represses mitochondrial gene expression and induces apoptosis in highly aggressive leiomyosarcoma cells.
    Article Snippet: Sigma-Aldrich, Hoechst 33258 (Phenol, 4-[5-(4-methyl1-piperazinyl)[2,5’-bi-1H-benzimidazol]-2’-yl]-, trihydrochloride) (Cat# 14530; CAS# 23491-45-4), IMT1B (LDC203974) ((S)-1-((R)-2-((4-(2-chloro-4-fluorophenyl)-2-oxo-2H-chromen-7-yl)oxy)propanoyl)piperidine-3-carboxylic acid), MedChemExpress (Cat HY-137067; CAS# 2304621-06-3).

    Staining:

    Article Title: Viral IL-10 regulates nasopharyngeal carcinoma cell progression via the JAK1/STAT3 signalling pathway
    Article Snippet: .. The paraformaldehyde was discarded, the cells were rinsed 3 times with 1×PBS, and then the cells in each well were stained with 1 mL of Hoechst 33258 (MedChemExpress, USA) staining solution for 15 min. ..



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    Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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    Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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    Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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    Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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    Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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    Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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    Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) Hoechst 33258 staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

    Journal: Poultry Science

    Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

    doi: 10.1016/j.psj.2026.106920

    Figure Lengend Snippet: Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) Hoechst 33258 staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

    Article Snippet: 300 μL of Hoechst 33258 dye solution (Beyotime Biotechnology, China) was added to each well to cover the cells, and the cells were stained in an incubator for 20 min (37 °C, 5 % CO 2 ).

    Techniques: Staining, Flow Cytometry